Composite

Part:BBa_K1878005:Design

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


fdhf/iRFP713


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 115
    Illegal XhoI site found at 451
    Illegal XhoI site found at 457
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 232
    Illegal NgoMIV site found at 714
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of this vector lacks a ribosome binding site.



Source

iRFP713 is a Rhodopseudomonas palustris bacterial phytochrome photoreceptor. The fdhf promoter was copied from the E. coli genome.


References